The objective of our study was to investigate changes in cell morphology and viability after sonoporation. Sonoportion was achieved by ultrasound (21 kHz) exposure on adherent human prostate cancer DU145 cells in the cell culture dishes with the presence of microbubble contrast agents and calcein (a cell impermeant dye). We investigated changes in cell morphology immediately after sonoporation under scanning electron microscope (SEM) and changes in cell viability immediately and 6 h after sonoporation under fluorescence microscope. It was shown that various levels of intracellular calcein uptake and changes in cell morphology can be caused immediately after sonoporation: smooth cell surface, pores in the membrane and irregular cell surface. Immediately after sonoporation, both groups of cells with high levels of calcein uptake and low levels of calcein uptake were viable; 6 h after sonoporation, group of cells with low levels of calcein uptake still remained viable, while group of c.
Download Free PDF View PDF
Journal of the Medical Sciences
Background: Gastric xanthelasmas are rare lesions. The lesions are frequently located in the stomach and less common sites are esophagus, duodenum and the colon. Case Presentation: We reported two cases in this study. A 50-year-old man with a past medical history of diabetes mellitus applied with dysphagia and upper gastrointestinal endoscopy revealed a yellow plaque (10×10mm) on the gastric mucosa of cardiac part and during biopsy is easily picked. Histopathological examination of the plaque excluded gastric cancer and revealed numerous large polygonal cells with abundant foamy cytoplasm. A 60-year-old man attended hospital with abdominal pain radiating to the back that there was a 3×2mm yellowish-white plaque in the antrum of the stomach. Histopathological examination of the gastric mucosa in the antrum showed mild chronic gastritis and some H.pylori-like microorganisms. Also, aggregates of foamy macrophages were presented. Conclusions: Although the clinical significance of gastri.
Download Free PDF View PDF
Surgical Case Reports
Background: Laparoscopic sleeve gastrectomy (LSG) progressively became the preferred procedure worldwide for the treatment of morbid obesity. Occasionally, unknown gastrointestinal diseases may be incidentally discovered during the procedure or on the histologic gastric specimen. Gastric xanthomas are uncommon lesions of the lamina propria, composed by foamy macrophages and mixed inflammatory infiltrate. Rarely, xanthoma cells develop within a gastric hyperplastic polyp. Although usually benign, they may be associated with pre-malignant conditions or even gastric cancer, making advisable an appropriate workup. Case Presentation: A hyperplastic polyp with xanthomatous proliferation was discovered in the gastric specimen of a young man, suffering from severe obesity and metabolic syndrome. The patient had been treated with proton pump inhibitors for gastric discomfort for years. After the surgical procedure, the gastric discomfort rapidly disappeared. Conclusion: Obesity is often comp.
Download Free PDF View PDF
